Implemented kill switches: 1
Kill Switch 1
Class
Linear line of repressors
Toxins
| Name | Number | Comment |
|---|---|---|
| MazF | K302033 | Coding toxic protein MazF |
Antagonists
| Name | Number | Comment |
|---|---|---|
| MazE | Not used here |
Topology
Parts
| Name | Number | Comment |
|---|---|---|
| IPTG | ||
| LacI | K1088018 | Coding lacI repressor from E. coli |
| CI | C0051 | Coding cI |
| Promoter 1 | J23116 | Weak constitutive promoter |
| Promoter 2 | R0010 | LacI repressible promoter |
| Promoter 3 | R0051 | CI repressible promoter |
| Double terminator | B0015 | Double terminator |